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Associate Professor Molecular Biology Genetics Work: Medical Research Center- Molecular Biology Department - Ph.D. degree in Microbiology2009. Subject of Study: “Molecular Comparative Study for the Evaluation ofDifferent Standardization Concepts in Quantitative Assay of Certain Infectious Microbial Disease. - M.S. degree inMicrobiology 2001. Subject of Study: “Clinical diagnosis of ocular infection of Chlamydia Trachomatis by polymerase chain reaction, a molecular approach.” - B.Sc. degree in Botany-chemistry, Faculty of science, Ain Shams University 1984. Applications Used in Both Study The studied samples were subjected to the following: Bioinformatics: 1-Sequence analysis and selection of PCR primers using gene bank and software. 2- DNA Synthesis, primer (Oligonucleotide) synthesis and purification. 3- Measurement of oligonucleotide concentration. 4- Extraction of nucleic acids from eye swabs by 6 different methods for evaluations. 5- End point amplification PCR amplification (Quantitative PCR by gel documentation system). 6- Detection of the amplified product and DNA analysis by agarose gel electrophoresis. 7-Real-time PCR. 8-Real-time NASBA 9-Primers and internal controls for the previous amplification methods were synthesized by DNA synthesizer, site directed mutagenesis and cloning technique. Cloning, DNA isolation from plasmid vectors (positive control). The nucleotide sequences of the DNA fragments inserted into the W and M plasmids were confirmed by direct sequencing in both strands FIELDS OF INTEREST: Molecular Diagnostic, Bioinformatics, microarray, Biotechnology, Gene expression. Sequencing, PCR quantitation,IsothermAmplification. To contact please email scofinan3@gmail.comTel.no. 01001290002. Publication 1-Interleukin 28B Polymorphism as a Predictive Factor to Treatment Response in Chronic Hepatitis C Genotype 4 Egyptian Patients Global Advanced Research Journal of Microbiology (ISSN:2315-5116) Vol.1(11) pp. 180-187, December,2012 2-The effect of cholinesterase inhibition on liver dysfunction in experimental acute liver failure The Egyptian Journal of Critical Care Medicine (2013) 1. 51-59 3- Sero-epidemiological profile of toxoplasma infected pregnant women in relation to repeated abortion at kasr Al-ainy obstetrics and gynaecology outpatient clinics in Cairo, Egypt Egypt. J. Med. Sci. Vol .36 (2), December 2015. 753-772. (ISSN:1110-0540) 4- Microscopic and molecular diagnosis of intestinal microsporidiosis among immunocompromized childrenThe Egyptian Journal of Medical Sciences Egypt. J. Med. Sci. 36 (2), December 2015: 809-820. (ISSN:1110-0540) 5- Genetic polymorphism of Toxoplasma gondii from recently infected aborted Egyptian women Journal of the Egyptian Society of Parasitology, Vol.46, No.1, April 2016 J.Egypt.Soc. Parasitol.(JESP).46(1).2016:47-53 6- Local Expression of T Cell Population In Sensitized And Unsensitized Experimental Hydatidosis. 2nd International Conference on Parasitology August 01-03, 2016 Manchester, UK 7- Toxoplasmosis and Abortion: Pro- and Anti-Inflammatory Cytokines Gene Expression of the Host Immune Cells. 2nd international Conference on Parasitology August 01-03, 2016 Manchester, UK. 8-Loop-Mediated Isothermal Amplification (LAMP): Sensitive and rapid detection of Schistosoma haematobium DNA in urine samples of Egyptian suspected cases. Journal of The Egyptian Society of Parasitology (JESP) 46(2),2016:299-308 9- Limit of molecular detection of Strongyloides stercoralis parasite performing two PCR protocols. Journal of the Egyptian Society of Parasitology, Vol.46, No.3, December 2016 J. Egypt. Soc. Parasitol. (JESP), 46(3), 2016:549 – 556 10- Resveratrolamelioratesoxidativestressandorgandysfunctionin Schistosomamansoni infectedmice ExperimentalParasitology174(2017)52- 58 11- Anti and pro-inflammatory cytokine gene expression in patients suffering from cancer bladder associated with chronic schistosomiasis haematobium The Egyptian Journal of Medical Science. Australian Journal of Basic and Applied Sciences, 11(1) January 2017, Pages: 55-61. 12- Evaluation of four DNA extraction methods seeking for successful amplification of S. haematobium genomic materials in urine samples from infectedcases. Volume 34(2), December 2013. 13- One minute, intraoperative assessment of the viability of hydatid cysts using a simple reagent strip test.HelminthologiaHE-2016-0047R4 2017/03/23. 14- QuantificationofViralLoad OF Hepatitis B and Hepatitis C Viruses inPlasma: Comparing Conventional PCR, real-time PCR and Isothermal NASBA. The NewEgyptian Journal of Microbiology Published by society forBiotechnology. Vol. 32, May, 2012 ISSN 1687- 1219
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