About us
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MASRI – tissue culture and stem cell research unit: started preparation, training of crew and working under supervision of the Italian tissue culture expert Silvia Porta 1996. The unit has been undergoing complete renovation, whether at the infrastructure level or at the hardware level 2019.
MASRI – tissue culture and stem cell research unit generally refers to the growth of cells from a tissue from a multicellular organism in vitro. These cells may be cells isolated from a donor organism, "primary cells", or an immortalized cell line. The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival.
MASRI – tissue culture and stem cell research unit Regarding the significance of regenerative medicine and stem cells therapy in the treatment of cancer, The tissue culture and stem cells Unit in the MASRI labs used the application of stem cells therapy using either various types of hepatocellular carcinoma cell lines, or by chemical induction of hepatocellular cancer in research animals with collaboration with Genomic Unit.
MASRI – tissue culture and stem cell research unit Isolation of stem cells from Bone marrow blood, Umbilical cord blood and Adipose tissues. We Maintain, proliferate and differentiate of mesenchymal stem cells into several types of cells according to growth factors added.
MASRI – tissue culture and stem cell research unit Transfection of somatic cells into induced pluripotent stem cells (IPCs) to reduce or avoid side effect of transplantation.
MASRI – tissue culture and stem cell research unit conduct different training courses to increase capacity building in new young researches.
MASRI – tissue culture and stem cell research unit is conducts some experiments on cancer cells to determine the effect of drugs used in various medical fields and Maintain different types of cells and changing of media, Cryopreservation of different types of cells by using liquid nitrogen.
Stakeholders
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Shadia A. Fathy a, Abdel Nasser B. Singab b, Sara A. Agwa c,Dalia M. Abd El Hamid c, Fatma A. Zahra d, Sawsan M. Abd El Moneim. The antiproliferative effect of mulberry (Morus alba L.) plant on hepatocarcinoma cell line HepG2. |
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Abd El Dayem, Samiha M.; Arafa, Mahmoud M.; Foda, Fatma M.A.; Helal, Mona A. M.; Abu Zahra, Fatma A. and Haggag, Nawal Z. (2015). Role of bone marrow mesenchaymal stem cells (MSCs) on restoration of fertility in male rats after exposure to endocrine disrupter. International Journal of Pharmacy Review & Research; 5 (3): 158-169. |
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El-Shennawy, Shahinaz F.; Abdel Aaty, Heba E.; Radwan, Nehal A.; Abdel-Hameed, Dina M.; Alam-Eldin, Yosra H.; El-Ashkar, Ayman M. and Abu-Zahra, Fatma A. (2015). Therapeutic potential of mesenchymal stem cells on early and late experimental hepatic schistosomiasis model. Journal of Parasitology, 101(5):587-597. |
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Elkahkya, Hanan O.; Fathya, Ghada; Abu-Zahra, Fatma A. and Afifya, Ahmed A. (2016). Autologous adipose-derived adult stem cell injection versus platelet-rich plasma injection in the treatment of rolling post acne scars. Journal of the Egyptian Women’s Dermatologic Society, 13: 165–172. |
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El Shakaaa, Nagwa M.; Elebiarya, Fayka H.K.; Saleh, Hanan A.; Abu Zahra, Fatma A. and Kamel, Mai T. (2016). Telocytes a new interstitial cell in the heart. immuhistochemical and electron microscopic study. Medical journal of Cairo university, 84 (2): 351-356. |
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Helal, Mona A.M., Shaheena, Noura E.M. and Abu Zahra, Fatma A. (2016). Immunomodulatory capacity of the local mesenchymal stem cells transplantation after severe skeletal muscle injury in female rats. Immunopharmacology and Immunotoxicology., 38 (6): 1-9. |
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Abd El Samad, Abeer A.; Raafat, Mona H.; Shokry, Y.; Abu Zahra, Fatma A. and Abdellah, A.M. (2016). Histological study on the role of bone marrow derived mesenchymal stem cells on sciatic nerve and gastrocnemius muscle in a model of sciatic nerve crush injury in albino rat. The Egyptian Journal of Histology, 38: 438-451. |
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Abdellah, Ahmed M.; Abu Aita, Nashwa A.; Amer, Hanaa A. and Abu Zahra, Fatma A. (2016). Effect of bone marrow mesenchymal stem cells on experimentally-induced liver fibrosis in rats. The Egyptian Journal of Comparative Pathology and Clinical Pathology, 29: 148-159. |
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Fathy, S.A.; Eman, M.A.; Abu Zahra, Fatma A.; Yossef, F.S. and Sami, R.M. (2016). The antiproliferative effect of Origanum majorana on human hepatocarcinoma cell line: suppression of NF-kB. . Cellular and Molecular Biology; 62 (10): 80-84. |
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Doreya M. Mahmoud, Manal S. Mahmoud1, Hayam M. Ezz-El-Din, Fatma A. Abo-Zahra,Rasha A ( 2016) . Effect on RH virulent and ME49 non-virulent strains of Toxoplasma gondii: in vitro and in vivo experimental studies. Meselhey1 Artesunate. Sci Parasitology; 17(3-4):83-92. |
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El-Nahrawy, Walaa A. M.; Abu Zahra, Fatma A. and El-Dab, Rabha E. A. (2017). Ameliorative role of bone marrow mesenchymal stem cells and rutin against acetaminophen induced hepatotoxicity in adult male rats. International Journal of Advanced Research, 5 (9): 450-459. |
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Hamed, Gehan M.; Seif, Ansam A.; Ahmed, Mona A.; Abu Zahra, Fatma A. and Nagib, Einas M. (2017).Effect of bone marrow-derived mesenchymal stem cells transplantation on hypertension in experimental diabetic nephropathy. Medical Journal of Ain Shams University, 84 (2): 251-256. |
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Adel El-Nazer,M.; El-Kholy, A.G.; Gomaa, M.F.; Abu Zahra, Fatma A. and Mostafa, Rania H. (2017).Autologous bone marrow-derived mesenchymal stem cells versus tension-free vaginal tape for treatment of female stress urinary incontinence: An open-label clinical trial. Medical Journal of Ain Shams University, 84 (2): 257-263. |
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Badawy, M.S.; El-fayomy, S.S.; Zaki, B.M.; Sayed, A.M. and Abu Zahra, Fatma A. (2018).Assessment of healing in Calvarial bone defect by allogenic demineralized bone matrix and adipose derived stem cells (Experimental study). Egyptian Journal of Plastic and Reconstructive Surgery, 42 (2): 353-362. |
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Atef, A.; Shaker, A.A.; Sadek, Eman Y.; Boughdadi, Nahed S.; Atalla, Suzi, S. and Abu Zahra, Fatma A. (2018)The optimal timing of adipose derived stem cells injection to improve skin flap survival in a rat model. European Journal of Plastic Surgery, 41 (4): 387–394. |
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Shaker, Raghda F.; El Gazzar, K.M.A.; Abdel Aal, A.M.; Abu Zahra, Fatma A.; Maher, Amany A. and Ahmed Elshahat, A. (2018).In vitro comparative study between mechanically emulsified fat grafts and enzymatically emulsified fat grafts. European Journal of Plastic Surgery, 41 (4): 377–386. |
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Ali, Shaymaa; Saad El-Din, Somaia A.; El Chamy, Hebt Alla; Badr, Takwa; Ghazal, Faten; Amer, Hanaa; Abu Zahra, Fatma A.; Kamel, Nadia and Abdalla, A. (2017).A clinicopathological study comparing the treatment effect of stem cell transplantation and antivascular endothelial growth factor (Bevacizumab) on adjuvant induced rheumatoid arthritis in rats joints.Stem Cell, 8 (4): 116-124. |
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Elkhateb, Lobna; Zohdy, A.; Atalla, Suzi S.; Moussa, Manal H.; Hamam, Ghada G. and Abu Zahra, Fatma A. (2018). Comparative study on acellular dermal graft versus propylene mesh both either loaded or unloaded with BM-MSCs in healing of skull bone defect in rats: Histological and immunohistochemical study.International Journal of Stem Cells, 11 (1): 1-11 |
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Shimaa Farouk ,Salwa Sabet ,Fatma A. Abu Zahra,Akmal A. El-Ghor (2018). Bone marrow derived-mesenchymal stem cells downregulate IL17A dependent IL6/STAT3 signaling pathway in CCl4-induced rat liver fibrosis. PlOS ONE https://doi.org/10.1371/journal.pone.0206130 Published: October 22, 2018 |
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Taha, AlShaimaa, Azza Atef, Fatma A. Abozahra, Doaa M. Youssef (2019). Infusion of bone marrow mesenchymal stem cells alleviates insulin resistance and initiates pancreatic regeneration in experimental type 2 diabetic-rats. , Egyptian Journal of Pure and Applied Science;57(1):19-31. |
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Youssryea Youssry, Somaia Ahmed Saad El-Din, Nadia Salah Kamel, Faten Ghazal, Mona Mahmoud,Mahmoud Mohamed, Hanaa Amer, Fatma Abd-Al Karim and Ahmed Abd-Allah (2019).A Clinicopathological Study Comparing the Therapeutic Role of the Mesenchymal Stem Cell and Bevacizumab as Anti Vascular Endothelial Growth Factor on the Lung as an Extra-articular Target in Adjuvant Induced Arthritis in Rats. Stem Cell 2019;10(1): 20-30. |
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Gehane M. Hamed, Wessam E. Morsy, Manal S. Abd-El Hamid, Arwa Abd el moniem Hassan, Fatma A. Abu Zahra (2019). Effect of Bone Marrow-Derived Mesenchymal Stem Cells on Ischaemic-Reperfused Hearts in Adult Rats with Established Chronic Kidney Disease. International Journal of Stem Cells;12:304-314. |
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Marwa. M. Hussein, Swelim. H. H, Hanaa A. Amer, Fatma A. Abu Zahra, Mourad M. Hadier(2019).Effect of Conditioned and Co Culture Media from The Umbilical Cord Mesenchymal Stem Cells on Heptoma Cell Line (Huh7). The Egyptian Journal of Hospital Medicine Vol. 77 (4), Page 5313-5321 |
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Manar Yehia Ahmeda, Mofida Mohammed Salaha, Samar Kamal Kassima, Amr Abdelaalb, Wael M. Elayata,⁎, Dalia Abdel-Wahab Mohameda, Amr El Foulyc, Fatma Abd Elkarim Abu-Zahra, (2019). Evaluation of the diagnostic and therapeutic roles of non-coding RNA and cell proliferation related gene association in hepatocellular carcinoma. Gene 706 .97–105 25. |
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Omayma M. Hassanin, Tarek M. El-Masry, Fatma A. Abu-Zahra, Sawsan El-Adawy, Ahmed Mohamed Abdellah (2019). Immune-Modulatory Changes After Transplantation Therapy of Insulin Producing Cells Derived from Wharton’s Jelly Human Umbilical Cord- Mesenchymal Stem Cells in Diabetes Induced Rats. The EGYPTIAN JOURNAL OF IMMUNOLOGY Vol. 26 (1), Page: 55-67. |
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Raghda Fayek Shaker 1, Abdel Rahman M Abdel Aal 1, Khaled Mohamed Ali El Gazzar 1, Fatma Abdel Karim Abu Zahra 2, Ahmed Elshahat. Eplasty(2020);In Vitro Comparative Study of Emulsified Fat Grafts. 20: e1. v.20; PMC718137 Published online 2020 Apr 17. |
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Einas Nagib Hussein1 , Gehane M. Hamed1, Ansam A. Seif1, Mona A. Ahmed1, and Fatma Abd Elkarim Abu Zahra. Effects of Mesenchymal Stem Cells Therapy on Cardiovascular Risk Factors in Experimental Diabetic Kidney Disease. Canadian Journal of Kidney Health and Disease Volume 7: 1–19. |
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Ayman Mohamed El-Ashkar1,2 ,Laila Mohammed El-Hosseiny1 ,Fatma e Abdelkarim Abu Zahra3 , Nermeen Mohammed Abd El-Samee1 , Ashraf M Mohammed Barakat4 , Shimaa Abdelraouf Elgohary5 , Abeer Fathy Badawy1(2020). Potential Therapeutic Effect of Allogenic Mesenchymal Stem Cells on Chronic Cerebral Murine Toxoplasmosis. Afro-Egyptian Journal of Infectious and Endemic Diseases · DOI: 10.21608/aeji.2020.23818.1051 . |
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Adel ElMissiry1, Fatma A Abuzahra2, Marwa ElMissiry3, Eman Shorub3, Reem Hashim3, Dalia A.M Mahmoud3 ( 2020). Association of angiotensin-converting enzyme gene polymorphism and clinical characteristics in Egyptian patients with major depressive disorder. Egyptian Journal of Psychiatary. Vol 42 ; Issue : 2;Page : 84-92. |
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Hesham El-Halaby, MD, Ayman El-Kahky, MD, Hesham Taha, MD, Fatma Abu-Zahra, MD, Ihab Nada, MD, Ahmed Saeed Mahmoud (2021). Role of Stem Cells in Treatment of Sensorineural Hearing Loss. QJM: An International Journal of Medicine, Volume 114, Issue Supplement_1, October hcab094.002, https://doi.org/10.1093/qjmed/hcab094.002 Published:01 October 2021. |
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Mohamed S. Othman1,2 · Azza M. Khaled1,3 · Amal H. Al Bagawi4 · Mohamed A. Fareid1,5 · Reda A. Hameed1,6 · Fatama A. Abu Zahra7 · Ahmed E. Abdel Moneim8(2022). Echinops spinosus effect against diabetes and its hepatorenal complications: total extract and favonoids fraction. |
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Riham Lashin, MD* Fatma A. Abu Zahra, PhD† Ahmed Elshahat, MD* Khaled Elgazzar, MD* (2022). Viability of Fat Cells in Frozen Fat Tissue in Relation to Thawing Technique.Received for publication March 31, 2022; accepted July 11, 2022. Copyright © 2022 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons. |
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An Ultra Structural Analysis Of The Huh7 Hepatocellular CarcinomaCell Line's Response To Conditioned And Co-Culture Media |
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A column graph illustrates elevation of number of publications through from 2009-2023 |
Research Topics
Running Research Topics: -
1. Exosomes isolated from bone marrow stem cell (BMSC) in treating in bone fractures in rats model
2. Effect of bone marrow stem cells ( BMSC ) in Wound healing in diabetes animal model Effect of selenium and nano selenium treated mesenchymal stem cell therapy for sepsis .
3. An experimental study comparing the effects of adipose derived stem cells and platelet rich plasma on wound healing in rats
4. Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells (BMSC) as a Treatment of Bone Fractures in Rats Model.
5. The therapeutic effect of using the stem cells for treating liver toxicity in rats.
6. Single Base Editing in Selected Disorders of Inborn Error of Metabolism.
The efficacy of Umbilical Cord Blood-derived Mononuclear Cells In Prevention Of Necrotizing Enterocolitis In Preterm Neonates.
Awards
Prof / Fatma Abd Elkareem Abu Zahra
* Editorial Board member of Journal of Embryology & Stem Cell Research (JES) ISSN: 2640-2637. March 2020.
* Official Nominator for the VinFuture Prize.jan 2024.
* Official reviewer of BMC Musculoskeletal Disorders journal(spring natur) 2024 Ref: Submission ID 02f111a6-5db6-49e8-948f-12472bff0330.
* Certificate of appreciation in honor of achievement I international publication that support Ain shams university world ranking Jul 2023,Jan 2023,Aug 2022,Jan 2022.
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| Dr/ Hanan Othman | |
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| Dr/Marwa Mohamad | Dr / Manal Ibrahim |
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Mawada Sharaf El din Mawada graduated from the Faculty of Science, Department of Biotechnology, Helwan University, and is a master’s student. She works with high efficiency in the field of tissue culture and extracting exosomes from stem cells. |
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Hamdy Imam (Technical Assistant) He graduated from the College of Nursing and has strong experience in cell culture, sterilization, and confronting any contamination problems in the laboratory. |
Projects
1- Name of Project: MATREX (MASRI CAncer Biobank and InTegrated Genomic solution AccRedited Center of Scientific EXcellence), STDF
Type of Project: Accredited Center of Scientific Excellence,
Role: Executive Director of MASRI Cancer Biobank
Project No: 41851.
2-Effective adjuvant therapy for liver cancer using exosomes derived from umbilical cord mesenchymal stem cells. faculty of Medicine and Science.(under evaluation)
3-Targeted Efferocytosis of Drug-Loaded Frozen Stem Cells: A Strategy to Regulate Macrophage Metabolism and Prevent Aging-related Bone Diseases (osteoporosis, osteoarthritis)
Egypt-China collaporation
Teaching and Training
1- The stem cells were isolated from the bone marrow with their cell-enhancing characteristics on the hemocytometer 6/2017.
2. Stem cells were isolated from umbilical cord blood and converted into osteoblast 10/2017.
3. Stem cells were isolated from fat cells and characterized by flow cytometry 20-21 April\2019
4. Stem cells were isolated from rat bone marrow, characterized and cell counted on a hemocytometer 8/2019.
5. Stem cells were isolated from Wharton`s jelly from the umbilical cord and their medical applications and uses were identified 5\2021.
6. Stem cells were isolated from the bone marrow of mice, characterized and counted on a hemocytometer August 2021.
7. Stem cells were isolated from umbilical cord blood and converted into bone cells 12/2021.
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Isolation of Mesenchymal stem cells from bone marrow of rate
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Join us
Prof Dr. Fatma Abou Zahra
Tel: 01227402746
Email: fatmaalzahraa@med.asu.edu.eg
Dr. Marwa.M.Hussien
Tel: 01210103878
Email: marwamohamed@med.asu.edu.eg